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Image Search Results
Journal:
Article Title: Lsh, an epigenetic guardian of repetitive elements
doi: 10.1093/nar/gkh821
Figure Lengend Snippet: Lsh targets to repetitive sequences. (A) Western analysis of Lsh expression in the embryonal carcinoma cell-line P19 and embryonal fibroblasts (MEFs) by using a specific antibody raised against the C-terminal portion of Lsh. (B) ChIPs were performed on P19 cells using the C-terminal antibody against Lsh or an IgG control. The precipitated material was examined by western analysis using the C-terminal anti-Lsh antibody for detection. (C) ChIPs were performed from P19 chromatin extracts using the C-terminal antibody against Lsh or an IgG control. Precipitated DNA or chromatin input was analyzed for the presence of the indicated repeat sequences. DNA was diluted (1:3) for the detection of linear PCR amplification.
Article Snippet:
Techniques: Western Blot, Expressing, Control, Amplification